PLANT DNA EXTRACTION PRACTICAL PROTOCOL

 By: N. Shreya Mohan (MSIWM042)         AIM– To isolate the DNA from a plant sample THEORY– Plant extractions for DNA is considered one of the tedious methods for high quality DNA isolations. Unlike animal tissues, which have the same tissue type in different species, plant tissues structural biomolecules and metabolites keep changing.Continue reading “PLANT DNA EXTRACTION PRACTICAL PROTOCOL”

LYMPHOCYTE CULTURE

BY: Ezhuthachan Mithu Mohanan (MSIWM043) Cells circulating in body and part of immune system are known as lymphocytes. There are mainly two main types of lymphocytes, which includes B Cells and T cells.  Peripheral Blood Lymphocyte culture:  PBLC is a technique used in Cytogenetics used for karyotyping analysis. The most convenient method to study chromosomesContinue reading “LYMPHOCYTE CULTURE”

AFFINITY CHROMATOGRAPHY

BY: SHAILY SHARMA (MSIWM041) Chromatography is a technique which is widely used for the separation of mixtures based on the physio-chemical differences between the stationary and the mobile phase of the chromatographic apparatus. The sample to be separated is dissolved in the mobile phase, which passes over the stationary phase (the immobile/fixed surface) and carriesContinue reading “AFFINITY CHROMATOGRAPHY”

GEL PERMEATION CHROMATOGRAPHY

BY: SHAILY SHARMA (MSIWM041) Chromatography, broadly, is a technique to separate two phases which are mutually immiscible. The phases are brought in contact with each other and one of these phases is stationary while the other phase is a mobile phase which moves over the surface of the stationary phase or percolates it. The interactionsContinue reading “GEL PERMEATION CHROMATOGRAPHY”

LOOP MEDIATED ISOTHERMAL AMPLIFICATION

BY: Reddy Sailaja M (MSIWM030) INTRODUCTION Infectious diseases affect living forms on earth that include but not limited to plants, animals including human beings. Pathogenic micro organisms i.e., bacteria, virus, parasites etc attack, hinder the growth and development of an organisms and sometimes lead to death. Infectious diseases that turn out to be pandemic haveContinue reading “LOOP MEDIATED ISOTHERMAL AMPLIFICATION”

CENTRIFUGATION

BY: Reddy Sailaja M (MSIWM031) Centrifugation is one of the most extensively used technique in research and development fields of biochemistry, molecular biology, biochemistry and pharmaceutical industries for varied applications like isolation of cells, fractionation of sub cellular particles and other macromolecules for analytical and clinical applications. Definition Centrifugation is a process of separation (orContinue reading “CENTRIFUGATION”

ION EXCHANGE CHROMATOGRAPHY

BY: Ria Fazulbhoy (MSIWM031) Principle Ion exchange chromatography is based on the principle of reversible exchange of ions and polar molecules by retaining the sample molecules on a column (inert support medium). It is based on electrostatic force of attraction or ionic interactions between the ions and the surface of the stationary phase which hasContinue reading “ION EXCHANGE CHROMATOGRAPHY”

AMES TEST- TEST FOR MUTAGENICITY

BY: RAHUL ANDHARIA (MSIWM001) Introduction: Mutagenic potential of any compound can be assessed by using AMES test. This test can be also called as test for mutagenicity (refers to compounds or substances causing mutations). Whether a given chemical can cause mutations in the DNA of test organism or not bacteria are utilized in this test.Continue reading “AMES TEST- TEST FOR MUTAGENICITY”

FLAGELLA STAINING

The motility of many bacteria is due to the presence of thread like appendages called flagella. Flagella are a thin proteinaceous structure which is originate from cytoplasm and comes out from the cell wall. Types of flagellar patterns: Monotrichous: single flagellum at one end. Lophotrichous: many flagella at one end. Amphitrichous: flagella on both ends.Continue reading “FLAGELLA STAINING”

DNA ISOLATION FROM BACTERIA

To Isolate Genomic DNA from Bacterial Cell Theory: In molecular biology the isolation and purification of DNA from cell is most important process. The bacterial cell that is used in this procedure should be grown in suitable media under favourable conditions and harvested in late log to early stationary phase. Along with DNA bacterial cellContinue reading “DNA ISOLATION FROM BACTERIA”